Organization of Wolbachia pipientis in the Drosophila fertilized egg and embryo revealed by an anti-Wolbachia monoclonal antibody
نویسندگان
چکیده
منابع مشابه
Organization of Wolbachia pipientis in the Drosophila fertilized egg and embryo revealed by an anti-Wolbachia monoclonal antibody
Cytoplasmic incompatibility (CI) in Drosophila is related to the presence of Wolbachia, an intracellular microorganism found in many species of insects. In order to study the intracellular localization of Wolbachia in eggs and embryos, we have purified the bacteria from fly embryos and subsequently generated a monoclonal antibody (Mab Wol-1) specific for Wolbachia. Indirect immunofluorescence s...
متن کاملLarge-Scale Identification of Wolbachia pipientis Effectors
Wolbachia pipientis is an intracellular symbiont of arthropods well known for the reproductive manipulations induced in the host and, more recently, for the ability of Wolbachia to block virus replication in insect vectors. Since Wolbachia cannot yet be genetically manipulated, and due to the constraints imposed when working with an intracellular symbiont, little is known about mechanisms used ...
متن کاملInvestigating the presence of Wolbachia pipientis in various mosquito species
Wolbachia pipientis are endosymbionts that infect many insects, arachnids, and nematodes. By comparing the Wolbachia DNA sequences contained in different infected insect species, one can investigate the evolutionary history of arthropods and the modes of transmission of this reproductive parasite. We collected and extracted DNA from several mosquito species to test for the presence of Wolbachia...
متن کاملPopulation Genomics of Infectious and Integrated Wolbachia pipientis Genomes in Drosophila ananassae
Coevolution between Drosophila and its endosymbiont Wolbachia pipientis has many intriguing aspects. For example, Drosophila ananassae hosts two forms of W. pipientis genomes: One being the infectious bacterial genome and the other integrated into the host nuclear genome. Here, we characterize the infectious and integrated genomes of W. pipientis infecting D. ananassae (wAna), by genome sequenc...
متن کاملDynamics of Wolbachia pipientis Gene Expression Across the Drosophila melanogaster Life Cycle
Symbiotic interactions between microbes and their multicellular hosts have manifold biological consequences. To better understand how bacteria maintain symbiotic associations with animal hosts, we analyzed genome-wide gene expression for the endosymbiotic α-proteobacteria Wolbachia pipientis across the entire life cycle of Drosophila melanogaster. We found that the majority of Wolbachia genes a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Mechanisms of Development
سال: 1995
ISSN: 0925-4773
DOI: 10.1016/0925-4773(95)00372-x